Cellular Imaging
State-of-the-Art Light and Electron Microscopy
The Cellular Imaging shared resource at Fred Hutch provides comprehensive microscopy services for biomedical and translational research including advanced light microscopy and electron microscopy. Our expert staff provides a complete workflow, including training for independent, 24/7 work in the core; assistance and technical support in microscopy techniques and their application; and tailored quantitative image analysis. We work with Fred Hutch users as well as external users from academia and industry.
Fred Hutch and Leica Microsystems have combined efforts to establish a Leica Center of Excellence in the Cellular Imaging core in support of a mission to drive new discoveries and insights from scientific research performed using the imaging systems.
Electron Microscopy
We offer consultation, training and specimen preparation for scanning and transmission electron microscopy, or SEM and TEM. Our equipment includes a 6610LV SEM instrument with high- and low-vacuum operation, a 120kV TEM instrument and a range of equipment for specimen processing.
Image by Eyayu Belay, Torok-Storb Lab, Fred Hutch
Light Microscopy
We offer a range of state-of-the-art equipment, knowledge and expertise in light microscopy, or LM. Our instruments include systems for incubator microscopy, automated image-based screening, super-resolution confocal microscopy, 3D light-sheet microscopy, and gel and blot imaging.
Image by Veronica Dave, Lund Lab and Prlic Lab, Fred Hutch
Image Analysis
Cellular Imaging offers support and training in image analytics with the deployment of multiple workstations equipped with open-source and commercial software dedicated to image processing, quantification and visualization. In addition, we also build personalized image analysis pipelines and algorithms for processing high-content datasets or complex imaging experiments.
Image by Andrea Doak, Cheung Lab, Fred Hutch
Our Team at Work
Our staff are experts in the use and application of technologies for electron and light microscopy in biomedical research. Our team provides comprehensive project consultation; completely assisted technical support in sample preparation, imaging and analysis; and personalized training to users who wish to become expert, independent microscopists.
TEM image of synaptic vesicles in a high-pressure frozen C. elegans. Magnification 6000x. Image by Yan Liu, Bai Lab, Fred Hutch
Stephen MacFarlane runs the electron microscope in the Cellular Imaging shared resource
Super-resolution image of MCF10A cells expressing EGFP-Utrophin, 100x objective. Image by Chris Simpkins, Cooper Lab, Fred Hutch
SEM of the head of a Drosophila melanogaster adult. 80X magnification. image by the Eisenman Lab, Fred Hutch
Drs. Peng Guo and Lena Schroeder work in the Cellular Imaging shared resource.
Super-resolution, single-slice micrograph of a wildtype larval salivary gland nucleus stained with antibodies to Lamin B (green) and dFz2C (red). Image by Dr. Susan Parkhurst, Parkhurst Lab, Fred Hutch
TEM image of synaptic vesicles in a high-pressure frozen C. elegans. Magnification 6000x. Image by Yan Liu, Bai Lab, Fred Hutch
Stephen MacFarlane runs the electron microscope in the Cellular Imaging shared resource
Super-resolution image of MCF10A cells expressing EGFP-Utrophin, 100x objective. Image by Chris Simpkins, Cooper Lab, Fred Hutch
SEM of the head of a Drosophila melanogaster adult. 80X magnification. image by the Eisenman Lab, Fred Hutch
Drs. Peng Guo and Lena Schroeder work in the Cellular Imaging shared resource.
Super-resolution, single-slice micrograph of a wildtype larval salivary gland nucleus stained with antibodies to Lamin B (green) and dFz2C (red). Image by Dr. Susan Parkhurst, Parkhurst Lab, Fred Hutch
How to Reach Us
