Cellular Imaging
State-of-the-Art Light Microscopy
The Cellular Imaging shared resource at Fred Hutch provides advanced light microscopy services for biomedical and translational research. Our expert staff provides a complete workflow, including training for independent, 24/7 work in the core; assistance and technical support in microscopy techniques and their application; and tailored quantitative image analysis. We also offer training and support for imaging and analyzing gels and blots. We work with Fred Hutch users as well as external users from academia and industry.
Fred Hutch and Leica Microsystems have combined efforts to establish a Leica Center of Excellence in the Cellular Imaging core in support of a mission to drive new discoveries and insights from scientific research performed using the imaging systems.
Light Microscopy
We offer a range of state-of-the-art equipment, knowledge, and expertise in light microscopy. Our instruments include systems for incubator microscopy, automated image-based screening, confocal microscopy, 3D light-sheet microscopy, and super-resolution microscopy.
Image by Veronica Dave, Lund Lab and Prlic Lab, Fred Hutch
Image Analysis
Cellular Imaging offers support and training in image analytics with the deployment of multiple workstations equipped with open-source and commercial software dedicated to image processing, quantification and visualization. We also build personalized image analysis pipelines and algorithms for processing high-content datasets or complex imaging experiments.
Image by Andrea Doak, Cheung Lab, Fred Hutch
Gel and Blot Scanning
We train and support gel and blot imaging and analysis using visible and infra-red wavelength scanners. Our equipment is well-suited for routine Western blot imaging and specialized fluorescence, chemiluminescence, and phosphor screen imaging.
Image by Cellular Imaging staff, Fred Hutch
Our Team at Work
Our staff are experts in the use and application of technologies for light microscopy in biomedical research. Our team provides comprehensive project consultation; completely assisted technical support in sample preparation, imaging and analysis; and personalized training to users who wish to become expert, independent microscopists.
Dr. Lena Schroeder works in the Cellular Imaging shared resource.
Stitched histology image of the rhesus macaque lymph node. Stained for hematoxylin (blue) and CD163 (brown), 10x objective. Sample provided by Dr. Karsten Eichholz, Corey Lab. Sample staining by Experimental Histopathology core, Fred Hutch. Image by Cellular Imaging staff, Fred Hutch
Super-resolution image of MCF10A cells expressing EGFP-Utrophin, 100x objective. Image by Chris Simpkins, Cooper Lab, Fred Hutch
Stitched confocal image of the human maternal-fetal interface. Fetal placental tissue anchored to and invading maternal decidua with fetal cytotrophoblasts (green) remodeling maternal spiral artery. Stained for DAPI (blue), CK7 (green) and Vimentin (red), 10x objective. Image by Dr. Caitlin DeJong, Prlic Lab, Fred Hutch
Dave McDonald runs a confocal microscope in the Cellular Imaging shared resource.
Super-resolution, single-slice micrograph of a wildtype larval salivary gland nucleus stained with antibodies to Lamin B (green) and dFz2C (red). Image by Dr. Susan Parkhurst, Parkhurst Lab, Fred Hutch
Dr. Lena Schroeder works in the Cellular Imaging shared resource.
Stitched histology image of the rhesus macaque lymph node. Stained for hematoxylin (blue) and CD163 (brown), 10x objective. Sample provided by Dr. Karsten Eichholz, Corey Lab. Sample staining by Experimental Histopathology core, Fred Hutch. Image by Cellular Imaging staff, Fred Hutch
Super-resolution image of MCF10A cells expressing EGFP-Utrophin, 100x objective. Image by Chris Simpkins, Cooper Lab, Fred Hutch
Stitched confocal image of the human maternal-fetal interface. Fetal placental tissue anchored to and invading maternal decidua with fetal cytotrophoblasts (green) remodeling maternal spiral artery. Stained for DAPI (blue), CK7 (green) and Vimentin (red), 10x objective. Image by Dr. Caitlin DeJong, Prlic Lab, Fred Hutch
Dave McDonald runs a confocal microscope in the Cellular Imaging shared resource.
Super-resolution, single-slice micrograph of a wildtype larval salivary gland nucleus stained with antibodies to Lamin B (green) and dFz2C (red). Image by Dr. Susan Parkhurst, Parkhurst Lab, Fred Hutch
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