Image Analysis
Visualize and Quantify Image Data
We provide four high-end workstations and support several image analysis packages for visualization, quantification and restoration of light microscopy image data. Our team offers tailored consultations on analysis of both light and electron microscopy images. With our resources, you can conduct quantitative data analysis on your own or fully supported by staff.
Schedule With Us
To learn more about image analysis services or to schedule a consultation, please contact:
Julien Dubrulle, Ph.D.
Morphometric and Image Analysis Scientist
Location: Arnold Building M1-A101
Office Hours: Tuesdays, 2-5 p.m.
Imaging workstations:
- Windows 10, 64-bit, Intel Xeon W-2295 3.0GHz 18 core, 512GB RAM, NVIDIA Quadro RTX 6000 24GB
- Windows 10, 64-bit, AMD Ryzen Threadripper 3990X 2.9GHz 64 core, 256GB, 2x GeForce RTX 2080 TI 11GB
- Windows 10, 64-bit, Intel i9 7900X / 3.30 GHz, 128 GB RAM, multiple data drives for storage
- Windows 7 SP1, 64 bit, Intel Xeon / 2.67 GHz, 48GB RAM, multiple data drives for storage
Supported software:
- Imaris
- Volocity
- AutoQuant
- TissueQuest and HistoQuest
- Nikon NIS Elements
- ImageQuant TL
- Li-Cor Image Studio
- Adobe Photoshop
- ImageJ/FIJI
- Leica LAS X
- Zeiss ZEN
- Cell Profiler
- MATLAB
Highlighted features:
- 3D reconstructions
- Quantify fluorescent intensities, shapes and volumes
- Deconvolve epi-fluorescent image data sets
- Track dynamics of moving objects
- Study interactions of multiple populations
- Convert 3D time-lapse data into movies
- Single particle analysis
We can perform analysis on virtually any input, including:
- Datasets: 2D, 3D, time-lapse, multi-well/high-throughput
- Image formats, both nonproprietary (e.g., .tif, .jpg, png) and commonly used biomedical formats (e.g., .dv, .lsm, .czi, .nd2)
- Samples: cultured cells, tissue sections, organoids, embryos, small organisms
- Labeling, including label-free (phase contrast, brightfield), fluorescence (IF, FISH, fluorescent proteins/dyes) and histochemistry (IHC, H&E, cell-type specific stains)
We apply custom-made, versatile image analysis pipelines to extract quantitative metrics related to:
- Cell/spot/object counting
- Subcellular localization, including location of subcellular structures and organelles, nucleocytopasmic ratio, and density of objects of interest in a cellular compartment
- Signal colocalization
- Morphometric analysis, including surface, volume, circularity, eccentricity, solidity and texture
- Spatial distribution, including position of cells, crowding or neighbor occupancy
- Cell tracking, including position, path and time-related metrics
- Wound healing assays
- High-throughput screening