Investigators can use HALO image analysis software with chromogenic and fluorescent images from a variety of imaging platforms. With this software, users can fuse single-channel images and tiles and register or overlay parallel images for simultaneous analysis. Users can also annotate images to denote separate layers for analysis or classifiers used to create annotated areas for analysis based on distinctive colors or textures. HALO offers gross area quantification or nuclear detection for cell quantification. Investigators can view the results of analysis as summary data or as cell-by-cell object data, export it to Excel for further analysis, or conduct further spatial/proximity analysis within HALO.
The following image analysis modules are available for use on the HALO workstations:
- Area quantification (chromogenic and fluorescent)
- Cytonuclear (chromogenic and fluorescent)
- Deconvolution
- FISH and ISH
- FISH-IF and ISH-IHC
- Multiplex IHC and Highplex FL
- Immune cell
- Membrane (chromogenic and fluorescent)
Remote use of the Halo analysis workstations, while using a VPN connection, is also supported.
We offer training for the independent use of our Aperio FL fluorescent slide scanner and HALO image analysis software, in addition to providing these services for a fee.
At the HALO online training portal, find tutorial videos, user guides, technical documentation, webinars and other learning resources for learning our HALO software.