We provide expertise in preparing and processing tissues for a wide range of routine and specialized procedures. If you have a custom request, we are available to consult with you to develop an innovative, workable solution to address your project's specific needs.
After tissue has undergone 10% neutral buffered formalin or PaxGene fixation, we process it on automated tissue processors and then embed it into paraffin blocks for slide or curl preparation. FFPE processing preserves tissue proteins and structures in a stable, easy-to-handle form, compatible with many types of assays.
When an assay requires unfixed specimens, we embed fresh tissue in OCT (optimal cutting temperature) medium and flash-freeze it in supercooled isopentane. This technique ensures the highest possible morphological preservation and minimizes freezing-related artifacts that can interfere with analysis.
Due to the preparation time required to process OCT frozen samples, please contact us at least 24 hours in advance to schedule your request.
Investigators can submit cells of all types for FFPE processing. We wash fixed pellets, centrifuge them, embed them in an agar-based matrix, and then process them identically to tissue. Sections of these pellets can be stained or undergo procedures such as immunohistochemistry and in situ hybridization.
Hematoxylin and eosin, or H&E, staining is a ubiquitous technique for visualizing cellular and whole-tissue morphology. This stain reveals the nucleus and cytoplasm of the cells, as well as extracellular components. Our Sakura Tissue-Tek Prisma autostainer is dedicated to high-volume H&E staining for fast turnaround.
When H&E staining alone does not provide adequate information, we can perform special tissue staining techniques. Special stains can confirm the presence or absence of specific tissue structures, cell types and microorganisms.
Our team is highly experienced in FFPE and frozen sectioning, and we can provide unstained tissue slides from FFPE or OCT blocks for your experiments. We also routinely prepare nuclease-free sections onto slides, or collected as curls, for downstream molecular and proteomic analysis.
Tissue microarrays, or TMAs, are comprised of tissue cores from multiple donor FFPE blocks and arranged as a mapped array in a new recipient paraffin block. TMAs can be used to compare normal vs. diseased tissues, or to perform screening across many tissue types on one slide, saving time and money. We can construct TMAs of up to 100 cores and prepare multi-tissue blocks containing larger pieces of tissue.
For training on our Leica laser capture microdissection, or LCM, instrumentation, contact our LCM Specialist David Sierra to schedule a session.