In the Comparative Medicine shared resource, our teams of technicians, veterinarians, pathologists and operations specialists offer comprehensive, high quality and compassionate services. We offer our time- and cost-effective services to both Fred Hutch and external users.
To schedule services with the Comparative Medicine shared resource, or to get more information about how we can work with you, visit our rates and scheduling page or send a message to the primary contact for the relevant service area, below.
For more information about the comparative medicine services we offer, contact Dr. Gordon Roble, senior director of comparative medicine and translational resources.
We offer consultation on animal model selection and development, experimental design, and veterinary, technical and care requirements prior to Institutional Animal Care and Use Committee (IACUC) protocol submission.
Our staff provides husbandry and veterinary care daily. We offer a wide variety of services, including colony management, basic and experimental procedure support, and monitoring. Our facility has dedicated surgery and procedure rooms.
The translational research model group within Comparative Medicine provides additional specialized technical services and preclinical models such as NSG, NSG-SGM3, NRG and NBSGW mice. For more information about translational research models, please contact Cassie Miller.
Fred Hutch investigators can learn more on CenterNet.
We maintain specific pathogen-free facilities through comprehensive testing and diagnostics and via preventive health services. Our optimal health services remove the potential for intercurrent disease to confound research data and, for some agents, to adversely affect well-being.
The preclinical imaging team provides state-of-the-art in vivo imaging technology and infrastructure to support basic and preclinical research. Our modalities include the following:
Additional imaging resources are available through the Translational Bioimaging Core, or TBIC, a partnership between our animal imaging team and the University of Washington Department of Radiology to support imaging research by members of the Fred Hutch/University of Washington Cancer Consortium.
We offer hands-on training to investigators in handling, equipment use, and a variety of techniques and procedures to aid in your research.
We provide diagnostic and research pathology expertise for a wide range of laboratory species. For more information on our comparative pathology services, contact Dr. Amanda Koehne, director of translational pathology.
For tissue processing, embedding and slide staining services, contact the Experimental Histopathology shared resource.
We provide a full range of pathology services including gross necropsy, microscopic interpretation and lesion scoring. We also offer consultation to investigators who are planning upcoming experiments involving histopathology.
Pathology and clinical veterinary staff collaborate through our biosecurity activities to ensure health. We can help determine the cause of any unexpected experimental results.
We offer hands-on trainings to individuals or small groups, tailored to attendees’ needs.
We support the development and maintenance of preclinical mouse models, including mice bearing a humanized immune system, and we provide access to high-quality technical services and immune-deficient mice. We can perform individual technical services or complete an entire in vivo experiment. For more information about our specialized mouse services, contact Cassie Miller.
We currently maintain various colonies, including NSG, NSG-SGM3, NRG and NBSGW mice, which are available for use in preclinical studies. For more information, contact Cassie Miller.
Human hematopoietic cells readily engraft into NSG and NRG; however, both myeloid and erythroid engraftment is limited. Transgenic expression of human cytokines (SCF, GM-CSF, IL-3) in NSG-SGM3 mice stimulates hematopoiesis, improves the growth and engraftment of hard-to-grow human leukemias and supports more robust and stable myeloid engraftment compared to NSG mice. Inactivating mutations in murine Kit in an NSG background (NBSGW) facilitates human hematopoietic cell engraftment without the need for preconditioning irradiation, and these mice display more robust myeloid and erythroid engraftment compared to NSG mice.
We currently offer three humanized mouse models — mice engrafted with human peripheral blood lymphocytes, human bone marrow or human hematopoietic CD34+ cells. Human bone marrow or CD34+ cells can be engrafted into either adult or neonatal mice.
Our basic analysis of human engraftment involves staining peripheral blood samples with a mixture of antibodies specific for mouse CD45, human CD45 (all human hematopoietic cells), human CD33 (myeloid cells), CD14 (monocytes, macrophages), CD19 (B cells) and CD3 (T cells). We can include additional antibodies where necessary and at users’ request. Our analysis can be longitudinal or at a discrete time point to determine success prior to downstream experiments.
We can conduct a wide array of individual technical services for translational studies, including, but not limited to, the following:
We can complete entire preclinical studies: from providing mice and grafting cells or tumors for study, to drug administration and downstream sample collection and analysis. We provide regular updates, including spreadsheets with data specific to the study. Contact us to discuss your needs and we will provide a customized study plan and cost estimate.