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Comparative Medicine Services

Enabling Research

In the Comparative Medicine shared resource, our teams of technicians, veterinarians, pathologists and operations specialists offer comprehensive, high quality and compassionate services. We offer our time- and cost-effective services to both Fred Hutch and external users.
 

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Comparative Medicine Services | Comparative Pathology Services | Translational Research Model Services

Schedule With Us

To schedule services with the Comparative Medicine shared resource, or to get more information about how we can work with you, visit our rates and scheduling page or send a message to the primary contact for the relevant service area, below.

Comparative Medicine Services

For more information about the comparative medicine services we offer, contact Dr. Gordon Roble, senior director of comparative medicine and translational resources.

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Assistance in Model Development and IACUC Protocol Preparation

We offer consultation on animal model selection and development, experimental design, and veterinary, technical and care requirements prior to Institutional Animal Care and Use Committee (IACUC) protocol submission.

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Research Support and Colony Management

Our staff provides husbandry and veterinary care daily. We offer a wide variety of services, including colony management, basic and experimental procedure support, and monitoring. Our facility has dedicated surgery and procedure rooms.

The translational research model group within Comparative Medicine provides additional specialized technical services and preclinical models such as NSG, NSG-SGM3, NRG and NBSGW mice. For more information about translational research models, please contact Cassie Miller

Fred Hutch investigators can learn more on CenterNet.

Example of a standard research procedure space.
Example of a standard research procedure space. Photo by Robert Hood / Fred Hutch News Service
Biosecurity

We maintain specific pathogen-free facilities through comprehensive testing and diagnostics and via preventive health services. Our optimal health services remove the potential for intercurrent disease to confound research data and, for some agents, to adversely affect well-being.

Preclinical Imaging

The preclinical imaging team provides state-of-the-art in vivo imaging technology and infrastructure to support basic and preclinical research. Our modalities include the following:

  • Ultra high-frequency ultrasound imaging
  • Bioluminescence and fluorescence optical imaging
  • 7.0 T cryogen-free magnetic resonance imaging
  • Multiphoton microscope for intravital imaging
  • Microcomputed tomography imaging

Additional imaging resources are available through the Translational Bioimaging Core, or TBIC, a partnership between our animal imaging team and the University of Washington Department of Radiology to support imaging research by members of the Fred Hutch/University of Washington Cancer Consortium.

Bioluminescence and fluorescence optical imaging equipment
Bioluminescence and fluorescence optical imaging equipment.
Comparative Medicine Training

We offer hands-on training to investigators in handling, equipment use, and a variety of techniques and procedures to aid in your research.

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Comparative Pathology Services

We provide diagnostic and research pathology expertise for a wide range of laboratory species. For more information on our comparative pathology services, contact Dr. Amanda Koehne, director of translational pathology.

For tissue processing, embedding and slide staining services, contact the Experimental Histopathology shared resource.

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Research Pathology

We provide a full range of pathology services including gross necropsy, microscopic interpretation and lesion scoring. We also offer consultation to investigators who are planning upcoming experiments involving histopathology.

Microscopic image
Normal intestine Image by Amanda Koehne
Diagnostic Pathology

Pathology and clinical veterinary staff collaborate through our biosecurity activities to ensure health. We can help determine the cause of any unexpected experimental results.

Pathology Training

We offer hands-on trainings to individuals or small groups, tailored to attendees’ needs. 

Translational Research Model Services

We support the development and maintenance of preclinical mouse models, including mice bearing a humanized immune system, and we provide access to high-quality technical services and immune-deficient mice. We can perform individual technical services or complete an entire in vivo experiment. For more information about our specialized mouse services, contact Cassie Miller.

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Immune-Deficient Mice

We currently maintain various colonies, including NSG, NSG-SGM3, NRG and NBSGW mice, which are available for use in preclinical studies. For more information, contact Cassie Miller.

Human hematopoietic cells readily engraft into NSG and NRG; however, both myeloid and erythroid engraftment is limited. Transgenic expression of human cytokines (SCF, GM-CSF, IL-3) in NSG-SGM3 mice stimulates hematopoiesis, improves the growth and engraftment of hard-to-grow human leukemias and supports more robust and stable myeloid engraftment compared to NSG mice. Inactivating mutations in murine Kit in an NSG background (NBSGW) facilitates human hematopoietic cell engraftment without the need for preconditioning irradiation, and these mice display more robust myeloid and erythroid engraftment compared to NSG mice.  

Humanized Mice

We currently offer three humanized mouse models — mice engrafted with human peripheral blood lymphocytes, human bone marrow or human hematopoietic CD34+ cells. Human bone marrow or CD34+ cells can be engrafted into either adult or neonatal mice.

Our basic analysis of human engraftment involves staining peripheral blood samples with a mixture of antibodies specific for mouse CD45, human CD45 (all human hematopoietic cells), human CD33 (myeloid cells), CD14 (monocytes, macrophages), CD19 (B cells) and CD3 (T cells). We can include additional antibodies where necessary and at users’ request. Our analysis can be longitudinal or at a discrete time point to determine success prior to downstream experiments.  

Humanized Mice
Technical Mouse Services

We can conduct a wide array of individual technical services for translational studies, including, but not limited to, the following:

  • Injections and implants through multiple routes
  • Irradiation, both focal and total body irradiation
  • Drug administration by injection or oral gavage
  • Noninvasive bioluminescence and fluorescence optical imaging
  • Sample collection
Mouse Experiments and Study Support

We can complete entire preclinical studies: from providing mice and grafting cells or tumors for study, to drug administration and downstream sample collection and analysis. We provide regular updates, including spreadsheets with data specific to the study. Contact us to discuss your needs and we will provide a customized study plan and cost estimate. 

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Questions about our services or how to schedule with us?