Our genetically engineered mouse model, or GEMM, program offers state-of-the-art services for the development and maintenance of transgenic mouse strains, including all modern genome editing tools and expertise in mouse transgenesis. We use CRISPR/Cas9 gene editing, which offers several advantages over traditional mouse transgenic technologies, including time savings, cost effectiveness and a potential reduction in animal usage. We continue to pursue new technologies and develop novel resources for preclinical research.
Our expert staff work with investigators from the first stages of experimental design through successful project completion.
We provide a four-phase CRISPR/Cas9 genome-editing service that allows researchers to acquire desired alleles (null, conditional, precisely mutated reporter or tagged alleles) in mice.
Phase I: Planning and preparation (2-3 weeks)
Phase 2: Gene editing and embryo transfer (1 week)
Phase 3: Tissue collection and genotyping (4 weeks)
Phase 4: Mouse selection (2 weeks)
We offer sperm cryopreservation services for effective mouse colony management. We collect and cryopreserve 75-100uL of sperm in each of 21 straws with cryopreservation media at two different cassettes in our tightly controlled facility. We will validate successful cryopreservation by motility analysis at no additional cost. Sperm motility does not guarantee viability. We also offer post-cryo quality assessment within 24 hours by in vitro fertilization of oocytes and progression to two-cell stage embryos.
Users must provide two healthy adult males as sperm donors (age 10-16 weeks, preferably with established breeding history). Cryopreservation success depends on the strain, transgenic mutations, age, breeding history and health of the donor male. Strains on common inbred backgrounds are most successful. Mice rederived from cryopreserved sperm will be heterozygous and/or wildtype.
We carry out in vitro fertilization of oocytes using cryopreserved or fresh sperm followed by embryo transfer surgery into the foster mother. We guarantee production of pathogen-free animals.
Included: Experimental consultation, donor and recipient females, all materials for IVF, superovulation of donor females, collection and transplantation of embryos into recipient females, ear/tail tissue collection and animal housing until weaning.
The adventitious pathogen status of a transgenic mouse line may impact breeding efficiency, health and life span and experimental variables such as immune response or microbiota. Adventitious agents are critical research confounders. We perform mouse strain rederivation to eliminate adventitious agents when they are identified during quarantine or routine murine health surveillance or as requested by researchers.
We thaw cryopreserved embryos and transfer them into pseudo-pregnant recipients via oviduct- or uterus-transfer surgeries.
Included: Experimental consultation, recipient females, collection and transplantation of resuscitated embryos into recipient females, ear/tail tissue collection and animal housing until weaning.
For those investigators who want to partially prepare their materials (plasmid, gRNA, etc.), the GEMM program also offers one-cell embryo pronuclear microinjections onto the desired strain background. We will ask clients to provide quality control data for all pre-produced materials. We only use C67Bl/6J embryos for pronuclear injection; however, we will alter the strain background based on client request.
We can help with the rapid expansion of an age-matched mouse colony via technically challenging IVF and embryo-transfer surgeries.
We can cryopreserve embryos from strains with unique genetic backgrounds or multiple genetic mutations requiring homozygosous frozen materials. We will work to meet all clients' needs.