To schedule antibody characterization services with the Antibody Technology shared resource, or to get more information about how we can work with you, fill out the inquiry form on our Rates and Scheduling page.
Using the iQue Mouse IgG Type and Titer assay kit, we can identify the IgG isotype, quantify IgG quantity per isotype, and assess cell viability and cell count.
Our Carterra LSA platform enables high-throughput monoclonal antibody characterization. Services include the following:
Compared to traditional Western blots, the Wes instrument from ProteinSimple enables faster, high-throughput, reproducible quantitation and analysis of proteins by size using only 3 μL of sample per capillary.
To provide researchers with essential sequence information for downstream antibody engineering, functional optimization and patent applications, we can sequence the antibody variable domain from mouse hybridoma cell lines, produce the recombinant antibody on mouse and human frameworks, and validate binding to the target antigen via flow-based ELISA.
If your objective is to identify new antibodies for immunohistochemistry applications (e.g., companion diagnostics), the Antibody Technology team works closely with the Experimental Histopathology shared resource to deliver and characterize novel antibodies that perform well for IHC.
Image from Balakrishnan A, Goodpaster T, Randolph-Habecker J, et al. Analysis of ROR1 protein expression in human cancer and normal tissues. Clin Cancer Res. 2017 23(12):3061-3071 doi: 10.1158/1078-0432.CCR-16-2083