Characterization Services

Antibody Characterization

Our antibody characterization services are available as the next step in our service pipeline after antibody discovery and antibody production, as well as for investigators who want to characterize antibodies from existing cell lines. 

Schedule With Us

To schedule antibody characterization services with the Antibody Technology shared resource, or to get more information about how we can work with you, fill out the inquiry form on our Rates and Scheduling page.

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Antibody isotyping

Using the iQue Mouse IgG Type and Titer assay kit, we can identify the IgG isotype, quantify IgG quantity per isotype, and assess cell viability and cell count.

Five graphs showing assay output
The Mouse IgG Type and Titer assay identifies the IgG isotype and measures IgG concentration with sensitivity ranging from 0.1 to 50 μg/mL. Image by Antibody Technology core staff Click for high-resolution image
High-throughput surface plasmon resonance

Our Carterra LSA platform enables high-throughput monoclonal antibody characterization. Services include the following:

  • High-throughput kinetic screening to determine the binding kinetics (ka and kd) and affinity (KD) of up to 1,152 monoclonal antibodies, 384 clones at a time in parallel, providing essential information for selecting optimal antibody candidates.
  • Epitope binning to characterize and group monoclonal antibodies by the epitope region to which they bind, providing important clues about functional inhibition. 
  • Epitope mapping of monoclonal antibody libraries against an immobilized array of 384 biotinylated peptides to identify the antibody’s binding site on the target antigen at the amino acid level.
  • Quantitation of up to 1,152 antibody and protein concentrations from purified samples and cell supernatants in a single assay. 
Photo showing the insertion tray in the Carterra LSA instrument.
Up to 384 immobilized ligands can be printed on the surface of Carterra LSA sensor chips, enabling high-throughput detection of binding interactions. Photo by Robert Hood / Fred Hutch News Service
Wes capillary electrophoresis

Compared to traditional Western blots, the Wes instrument from ProteinSimple enables faster, high-throughput, reproducible quantitation and analysis of proteins by size using only 3 μL of sample per capillary. 

Wes protein blot output image showing gray and black bands
Seven mouse antisera profiled for binding to target proteins by WES protein blot. Image by Antibody Technology core staff
Variable domain sequencing

To provide researchers with essential sequence information for downstream antibody engineering, functional optimization and patent applications, we can sequence the antibody variable domain from mouse hybridoma cell lines, produce the recombinant antibody on mouse and human frameworks, and validate binding to the target antigen via flow-based ELISA.  

In silico validation for a "productive" antibody gene sequence.
In silico validation for a "productive" antibody gene sequence. Image by Hieu Do, Antibody Technology core Click for high-resolution image
Immunohistochemistry

If your objective is to identify new antibodies for immunohistochemistry applications (e.g., companion diagnostics), the Antibody Technology team works closely with the Experimental Histopathology shared resource to deliver and characterize novel antibodies that perform well for IHC.

Image from Balakrishnan A, Goodpaster T, Randolph-Habecker J, et al. Analysis of ROR1 protein expression in human cancer and normal tissues. Clin Cancer Res. 2017 23(12):3061-3071 doi: 10.1158/1078-0432.CCR-16-2083

Microscopic image of cells
Representative IHC images of ROR1 staining in different regions of the macaque gastrointestinal tract. Scale bar represents 100 μm. Regions in squares in middle panels are magnified 10× in bottom panels. Click for high-resolution image
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Questions about our antibody characterization services or how to schedule with us?