Antibody Technology

Monoclonal Antibody Discovery for Research, Therapeutics and Diagnostics

Since our inception in 1995, Fred Hutch’s Antibody Technology shared resource core has worked with research teams inside and outside the Hutch to design campaign strategies for the generation and identification of novel monoclonal antibody compositions. Our projects encompass a range of applications in oncology, viral therapeutics and diagnostics.
 

We offer competitive rates for antibody technology services.

See Our Rates
photo inside a robotic system in a scientific setting

Antibody Discovery

We offer full-service hybridoma antibody discovery through mouse and rat immunization. We have experience immunizing a number of different immunogens including: hapten and peptide conjugates, proteins, mRNA and cellular immunizations.

Discovery Services
closeup photo of tubing and valves

Antibody Production

We offer scalable productions from hybridoma cultures ranging from 5 mg to 2 grams of research grade antibody.  We also offer recombinant antibody expression from Expi293 expression system.

Production Services
photo of a portion of machinery in a laboratory showing wires and small tubes

Antibody Characterization

Our characterization services include isotyping, variable domain sequencing, binding kinetics and epitope binning using surface plasmon resonance.

 

Characterization Services

Our Team at Work

We work with researchers to meet each project’s specific objectives. Guided by our team’s combined experience and our commitment to scientific excellence, we will assist you in designing and executing the appropriate strategy for producing the reagent you need.

Epitope mapping is performed using high throughput SPR using Carterra LSA instrument

Epitope mapping is performed using high throughput SPR using Carterra LSA instrument

Frozen archive of hybridomas are stored in liquid nitrogen

Frozen archive of hybridomas are stored in liquid nitrogen

Epitope mapping is performed using high throughput SPR using Carterra LSA instrument

Epitope mapping is performed using high throughput SPR using Carterra LSA instrument

Frozen archive of hybridomas are stored in liquid nitrogen

Frozen archive of hybridomas are stored in liquid nitrogen

Hybridoma colony picking using ClonePix 2 colony picker.

Hybridoma colony picking using ClonePix 2 colony picker.

Hybridoma supernatants are screened for antigen specificity using high throughput flow cytometry with iQue Screener instrument.

Hybridoma supernatants are screened for antigen specificity using high throughput flow cytometry with iQue Screener instrument.

Hybridoma colony picking using ClonePix 2 colony picker.

Hybridoma colony picking using ClonePix 2 colony picker.

Hybridoma supernatants are screened for antigen specificity using high throughput flow cytometry with iQue Screener instrument.

Hybridoma supernatants are screened for antigen specificity using high throughput flow cytometry with iQue Screener instrument.

40

Campaigns Annually
These campaigns span antibody discovery, production and characterization.

7500

Hybridoma Lines
Many lines we’ve developed are under patent filings or used commercially.

>50

Licensed Lines
Dozens of hybridoma cell lines we’ve developed have been licensed.

How to Reach Us

Eric Tam

Eric Tam

Director, Antibody Technology Shared Resource

For service requests or other questions: Please submit an inquiry.

206.667.4207

E1-309