Production Services

Antibody Production

Our antibody production services are available as the next step in our service pipeline after antibody discovery or for previously established hybridoma clones. 

Schedule With Us

To schedule antibody production services with the Antibody Technology shared resource, or to get more information about how we can work with you, fill out the inquiry form on our Rates and Scheduling page.

Submit an inquiry form for services

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Hybridoma subcloning

Primary "target-positive" hybridoma clones are archived and the secreted antibodies are analyzed for their performace in specific appications. Once the leads are identifed, we carry the primary clones through a subcloning and validation process. These final clones are archived as eight validated subclones from the parental line.

Automated supernatant harvest and cell freeze of primary clones prior to subcloning on the ClonePix II.
Automated supernatant harvest and cell freeze of primary clones prior to subcloning on the ClonePix II. Image by Antibody Technology Core staff
Antibody production and affinity purification

We affinity purify monoclonal antibodies from hybridomas cultured in serum-free media. Our typical threshold for antibody production is 200 mg of research-grade and preclinical material. We currently offer affinity purification at micro (10-80 μg), small, medium (2-25 mg) and large (25-200 mg) scales, or at a custom scale based on your needs.

Graph of chromatography output
Our automated chromatography system combines tandem affinity purification followed by a buffer exchange, enabling one-step purification of antibodies from culture supernatants. Click for high-resolution image
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Questions about our antibody production services or how to schedule with us?