The Thermo Scientific Orbitrap Eclipse combines quadrupole, OrbiTrap and ion trap mass analyzers into a single instrument, called a Tribrid design, that provides high data acquisition flexibility and utility. The Orbitrap analyzer can obtain data at resolutions ranging from 7,500 to 500,000 (at m/z 200) with mass accuracy of <3 ppm. The unique design and data acquisition strategies allow the Eclipse to acquire data at 45 Hz with the ion trap and 40 Hz with the Orbitrap. A field asymmetric ion mobility spectrometry, or FAIMS, unit is also mounted on the Eclipse. This provides gas-phase ion mobility separations to increase the signal to noise ratio of ions entering the mass spectrometer and to increase overall data quality.
Like the older generation Fusion, the Eclipse is equipped with synchronous precursor selection, or SPS, for selecting MS2 precursors for MS3 analysis to decrease the effects of precursor co-isolation during MS1, resulting in increased quantification accuracy for TMT and iTRAQ experiments. The Eclipse is also equipped with real-time database searching to increase the utility and success rate of MS3 quantification scans. A Thermo Scientific Easy-nLC 1200 UHPLC is coupled to the Fusion to provide liquid chromatography mass spectrometry, or LC-MS, at flow rates of 200 nL/min. to 800 nL/min. and at either conventional or ultrahigh-pressure liquid chromatography conditions.
Recommended uses: This system is primarily used for protein identification and quantification (SILAC, TMT, iTRAQ, label-free, spectral counting) of samples with very high complexity (e.g., lysates) and for global protein modification characterization (phosphoproteomics).