Metabolomics Services
Metabolite Profiling and Quantification
We have dedicated mass spectrometry instruments for metabolomics services, including assays for profiling and quantifying metabolites in a variety of sample types. We also offer expert consultation and services in sample preparation and data analysis.
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Schedule With Us
To schedule metabolomics services or to get more information about how we can work with you, view our rates and scheduling page or contact our core director:
Dr. Phil Gafken
Director, Proteomics & Metabolomics shared resource
The Thermo Scientific Q Exactive HF-X mass spectrometer combines a quadrupole mass filter in-line with a high-field Orbitrap mass analyzer. The Orbitrap can obtain data at resolutions ranging from 7,500 to 240,000 (at m/z 200) and mass accuracies of < 3 ppm. When acquiring data at the 7,500-resolution setting, the Orbitrap can acquire data at 40 Hz. Polarity switching at <1 sec. is possible, and the instrument is equipped with higher-energy collision-induced dissociation, or HCD, for carrying out tandem mass spectrometry analyses. A Thermo Scientific Vanquish Horizon HPLC with autosampler is coupled via a heated electrospray ionization, or HESI, source to the HF-X. Typical flowrates during LC-MS analysis range between 50 µL/min. to 300 µL/min.
Recommended uses: This system is used for untargeted profiling of polar metabolites using hydrophilic interaction chromatography, or HILC, and phospholipids and fatty acids using C18 reverse phase chromatography. We develop analyses for additional metabolite classes when requested.