Flow Cytometry: How We Work

Collaborating with You to Advance Your Science

Our staff are here to aid in use of a wide array of cytometers. We consult at every step of the process, including panel design, and marker and color choice, as well as data analysis and troubleshooting. However, we do not independently analyze samples for users.

We are the only flow cytometry facility in the Pacific Northwest that offers therapeutic cell sorting, and collaborate closely with Fred Hutch’s Therapeutic Products Program to support investigators administering early-stage clinical trials of cellular immunotherapies.

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Flow cytometry staff assisting flow cytometry core user. Photo by Robert Hood / Fred Hutch News Service
CASE STUDY

Revealing the Immune Response to CAR-T Cell Therapy

The Flow Cytometry core helped Dr. Sylvain Simon design a flow panel and standardized assay to examine how CAR-T therapy affects patients’ immune cells.

Training

During business hours, we have trained and knowledgeable staff available to assist users with using the cytometers, setting up an experiment, and data analysis. Therefore, users do not need to be trained to use our cytometers during business hours.

If users are interested in using cytometers after business hours, training is required. Currently, we do not offer formal training classes on the use and maintenance of the cytometers. However, we do offer personal one-on-one training. This training may involve startup, shutdown, and/or troubleshooting. Please contact the flow cytometry staff for more details.

For more information on the multiple aspects of flow cytometry, please refer to the following resources:

BD Biosciences Web-Based Training
ThermoFisher Scientific flow cytometry resources

Frequently Asked Questions

How do I make a reservation on the Flow Cytometry core’s cytometers?

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Both internal and external users can reserve cytometers in the Flow Cytometry core through iLab.

Can I drop my samples off for analysis?

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We do not offer independent analysis of samples for users. 

Do I need training to use a cytometer?

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You do not need to be trained on how to use a cytometer to make a reservation for assisted use during normal business hours. If you are interested in receiving hands-on training to use the instruments after-hours, please contact the Flow Cytometry Staff for more information.

I was not expecting the results that I obtained from one of the core’s cytometers. What can I do?

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Please contact the Flow Cytometry core staff for assistance with troubleshooting your unexpected results.