Flow Cytometry and CyTOF Services
Cutting-Edge Flow Cytometry and Mass Spectrometry
Our flow cytometry core offers internal and external users a wide array of cytometers for accurate and reliable cell analysis, including fully sterile cell sorting for therapeutic applications. Users can also obtain high-parametric data with the CyTOF technology in the core’s Fluidigm Helios mass cytometer. Our experienced and knowledgeable staff offers in-depth, hands-on training and consultation to all core users.
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Schedule With Us
To schedule services with Flow Cytometry, or to get more information about how we can work with you, contact our labs:
Phone (Thomas Building): 206.667.6195
Phone (Steam Plant Building): 206.667.1221
We have a broad range of advanced cell analyzers from BD Biosciences and Sony Biotechnology. All our cell analyzers can acquire data from tubes and multi-well plates, with the option of high-throughput analysis.
Our team can provide you with consultation, training, assay development or assisted sessions.
Our team can provide you with consultation, training, assay development or assisted sessions.
We offer advanced cell analyzers from BD Biosciences, Sony Biotechnology and Miltenyi Biotec that allow for live-cell sorting of a variety of cell types.
Cell sorting is considered a laboratory procedure hazard due to its potential for aerosol production. Therefore, pathogens designated as Risk Group 2 and higher may not be handled in this facility unless fixed by methods verified as effective against the infectious agent.
The Sony ID 7000F is a high-parameter spectral cell analyzer with seven lasers and 186 detectors, making it one of the most advanced cytometers in the world. By collecting the entire spectrum of light from 360 nm to 920 nm, this cutting-edge cytometer allows for accurate detection of dim and rare cell populations with high sensitivity.
The BD FACSymphony A5 is an innovative and customized cell analyzer with five lasers allowing up to 28 colors simultaneously. This cytometer enables users to analyze complex fluorochrome panels with high accuracy. Two of our instruments, Symphony 3 and Symphony 5, can also be configured for spectral analysis (SE), which allows for more flexibility in your panel design.
The BD LSRFortessa X-50 is an innovative and customized cell analyzer with five lasers allowing up to 28 colors simultaneously. This specialized cytometer also can be configured for spectral analysis, which allows for more flexibility in your panel design.
The BD FACSCelesta is a custom cell analyzer with four lasers allowing up to 16 colors simultaneously. This powerful, compact instrument allows for a large panel while maintaining ease of use.
The BD Canto II is a reliable cell analyzer with three lasers allowing up to 8 colors simultaneously.
The Cytek Aurora is a full spectrum high parameter cell analyzer equipped with 5 lasers and 64 detectors. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity.
The BD FACSymphony S6 is an innovative and customized cell sorter with five lasers allowing up to 28 colors simultaneously. This cell sorter allows for six-way sorting and into variously sized tubes and multi-well plates. This device has spectral capabilities, please contact our team for more information.
The BD Aria II is a cell sorter with up to five lasers allowing up to 18 colors simultaneously. This cell sorter allows for four-way sorting and into variously sized tubes and multi-well plates.
The Sony MA900 is a cell sorter is an innovative and intuitive cell sorter with four lasers allowing up to 12 colors simultaneously. This cell sorter is notable for its ease of use even for less experienced users.
The Miltenyi Biotec MACSQuant Tyto is a cell sorter with three lasers and 10 detectors allowing for 8 color detection. This unique instrument allows users to quickly and gently sort cells in a fully sterile environment.
Data Analysis
The Flow Cytometry core does not provide licenses for data analysis software packages, such as FlowJo. For Fred Hutch employees, FlowJo is available at the computer center within the Arnold Library.
If a user needs assistance in troubleshooting unexpected results collected on our instruments, please contact the flow cytometry core staff for more information.