Flow Cytometry and CyTOF Services

Cutting-Edge Flow Cytometry and Mass Spectrometry

Our flow cytometry core facility offers internal and external users a wide array of cytometers for accurate and reliable cell analysis, including fully sterile cell sorting for therapeutic applications. Users can also obtain high-parametric data with the CyTOF technology in the core’s Fluidigm Helios mass cytometer. Our experienced and knowledgeable staff offers in-depth, hands-on training and consultation to all core users.

ON THIS PAGE

Services  |  Cell Analyzers  |  Cell Sorters  |  CyTOF Services

Schedule With Us

To schedule services with Flow Cytometry, or to get more information about how we can work with you, contact our labs:

Phone (Thomas Building): 206.667.6195
Phone (Steam Plant Building):
206.667.1221

Services

Expand All
Conventional Flow Cytometric Analysis

We have a broad range of advanced cell analyzers from BD Biosciences and Sony Biotechnology. All our cell analyzers can acquire data from tubes and multi-well plates, with the option of high-throughput analysis.

Our team can provide you with consultation, training, assay development or assisted sessions. 

Conventional Flow Cytometric Analysis
Image by the Flow Cytometry shared resource
Spectral Flow Cytometric Analysis

Our team can provide you with consultation, training, assay development or assisted sessions.

PerCP-Cyanine5.5 Graph
Image by ThermoFisher Click for high-resolution image
Live-Cell Sorting

We offer advanced cell analyzers from BD Biosciences, Sony Biotechnology and Miltenyi Biotec that allow for live-cell sorting of a variety of cell types.

Lab member in Flow Cytometry shared resource
Photo by Robert Hood / Fred Hutch News Service
Lorem ipsum

Cell Analyzers

Expand All
Sony ID 7000F

The Sony ID 7000F is a high-parameter spectral cell analyzer with seven lasers and 186 detectors, making it one of the most advanced cytometers in the world. By collecting the entire spectrum of light from 360 nm to 920 nm, this cutting-edge cytometer allows for accurate detection of dim and rare cell populations with high sensitivity. 

Sony ID 7000F Instrument
BD FACSymphony A5

The BD FACSymphony A5 is an innovative and customized cell analyzer with five lasers allowing up to 28 colors simultaneously. This cytometer enables users to analyze complex fluorochrome panels with high accuracy.

BD FACSymphony A5
BD LSRFortessa X-50

The BD LSRFortessa X-50 is an innovative and customized cell analyzer with five lasers allowing up to 28 colors simultaneously. This specialized cytometer also can be configured for spectral analysis, which allows for more flexibility in your panel design. 

BD LSRFortessa X-50
BD FACSCelesta

The BD FACSCelesta is a custom cell analyzer with four lasers allowing up to 16 colors simultaneously. This powerful, compact instrument allows for a large panel while maintaining ease of use.

BD FACelesta Instrument
BD Canto II

The BD Canto II is a reliable cell analyzer with three lasers allowing up to 8 colors simultaneously.

BD Canto II Instrument
BD LSR II

The BD LSR II is a cell analyzer with four lasers allowing up to 18 colors simultaneously.

BD LSR II Instrument

Cell Sorters

Expand All
BD FACSymphony S6

The BD FACSymphony S6 is an innovative and customized cell sorter with five lasers allowing up to 28 colors simultaneously. This cell sorter allows for six-way sorting and into variously sized tubes and multi-well plates.

BD FACSymphony S6
BD Aria II

The BD Aria II is a cell sorter with up to five lasers allowing up to 18 colors simultaneously. This cell sorter allows for four-way sorting and into variously sized tubes and multi-well plates.

BD Aria II Instrument
Sony MA900

The Sony MA900 is a cell sorter is an innovative and intuitive cell sorter with four lasers allowing up to 12 colors simultaneously. This cell sorter is notable for its ease of use even for less experienced users.

Sony MA900 Instrument
Miltenyi Biotec MACSQuant Tyto

The Miltenyi Biotec MACSQuant Tyto is a cell sorter with three lasers and 10 detectors allowing for 8 color detection. This unique instrument allows users to quickly and gently sort cells in a fully sterile environment.

Miltenyi Biotec MACSQuant Tyto Instrument

CyTOF Services

Expand All
Fluidigm Helios CyTOF System

The Fluidigm Helios CyTOF mass spectrometry system enables analysis of more than 40 cell surface and intracellular markers without the spectral overlap seen in conventional flow cytometers. Learn more by downloading the user guide below.

Data Analysis

The Flow Cytometry core does not provide licenses for data analysis software packages, such as FlowJo. For Fred Hutch employees, FlowJo is available at the computer center within the Arnold Library.

If a user needs assistance in troubleshooting unexpected results collected on our instruments, please contact the flow cytometry core staff for more information. 

Questions about our Flow Cytometry services or how to schedule with us?

Last Modified, January 19, 2021