Our core aims to empower users to collect and process their own cryo-EM data. We train users to operate two different transmission electron microscopes, the Talos L120C and the Glacios. Users will learn how to screen grids and collect high-resolution cryogenic data, as well as how to process their data using the high-performance computing cluster maintained by Fred Hutch's Center IT.
The core provides access to single-tilt grid holders for room-temperature and cryogenic applications using the Talos microscope.
Temporary data storage is available for one month after data collection. On-the-fly motion correction and pre-processing are provided with Glacios reservations. We encourage users to consult with core staff during all stages of project development.
To request training or services, please refer to our rates and scheduling page.
We provide structured training in single-particle electron microscopy analysis for both experienced and new users. Core staff will work with researchers to set up negative-stain data collection and cryogenic screening on the Talos L120C microscope. We offer training in cryo-EM screening and large-dataset collection for single-particle projects on the Glacios microscope.
We will grant users independent-use status and door access to the microscope rooms after they pass proficiency tests at the end of each training section.
Our staff will collect a full dataset on prepared negative-stain grids using data-collection parameters that we have decided and agreed upon during consultation with users. We will provide micrographs to users immediately following collection.
Our staff will collect and process negative-stain data up to the point of generating 2D class averages to assess particle size, morphology and homogeneity. We will provide class averages less than four business days after we complete collection.
These image-processing resources may help shared resource users expand their cryo-EM knowledge:
This transmission electron microscope, or TEM, from Thermo Fisher Scientific is operated at an accelerating voltage of 120 kV and is equipped with a 4k x 4k CMOS detector that can record data at up to 40 frames per second. The microscope is fitted with a long-duration dewar that allows for up to four days of uninterrupted microscope usage, enabling fully automated overnight data collection. This microscope and detector support negative-stain and cryo-EM screening and data collection.
Trained users will have access to:
This TEM from Thermo Fisher Scientific is operated at an accelerating voltage of 200 kV and is equipped with a 24 megapixel (5,760 x 4,092) Gatan K3 direct electron detector which can record data at 1,500 frames per second. The Glacios is used for screening and high-resolution data collection of cryo-EM samples. The 12-grid cryo-autoloader allows for efficient and reliable screening of multiple samples and the XFEG electron source offers a bright and coherent electron beam for high-resolution images.
Users will have access to:
Available for users during their data collection sessions on the Glacios/K3, this machine runs Warp EM for on-the-fly motion correction and preprocessing, including contrast transfer function (CTF) estimation, particle picking and 2D classification.
This storage server will be available for one month of temporary data storage for all data generated during a data-collection session, including raw movies and aligned micrographs.