Our experienced Electron Microscopy team provides investigators with state-of-the-art technologies and expertise. We offer consultation, full service specimen preparation, and training in transmission and scanning electron microscopy, or TEM and SEM. We encourage an initial intake meeting and ongoing open communication with our staff to ensure your EM imaging meets your project goals. We also welcome all users to train with us to use the microscopes independently.
To schedule TEM and SEM services with the Electron Microscopy shared resource, or to get more information about how we can work with you, contact our electron microscopy team:
Associate Director, Electron Microscopy
We provide users with high-resolution, high-magnification TEM images of cellular and sub-cellular structures from fixed, embedded material. Fine external details can also be quickly viewed by TEM using negative staining techniques. Individual investigators may conduct imaging on the microscope, or our staff can provide imaging services.
Examples of specimens imaged by TEM include:
All scale bars, 500 nm.
We offer the following TEM imaging services:
We offer the following specimen processing services for TEM:
Our ThermoFisher Talos L120C transmission electron microscope has the following features:
Our Leica EMUC7 ultramicrotomes are capable of ultrathin sectioning (30 to 150 nm) and semithin sectioning (0.2 to 2 µm).
Our Leica VT1200S vibratome is a fully automated vibrating blade microtome for sectioning fresh or fixed tissue with the following features:
Our Leica EMPact2 high-pressure freezer vitrifies samples up to 200 µm in thickness. Other features include the following:
Our Denton benchtop turbo coating system has the following capabilities:
Our Beckman Airfuge offers small-volume, air-driven centrifugation.
We provide one-on-one training for users interested in learning to operate the Talos L120C TEM independently. We review room temperature sample loading, focusing and image acquisition using manual acquisition TIA software from Thermo Fisher. If a more automated workflow is desired, facility staff can work with users to develop a semi-automated data collection protocol using SerialEM. Users can schedule training on our iLab site as assisted use time on the TEM. We grant users who have been cleared as independent by EM shared resource staff 24/7 access to our microscope. Users must be trained and cleared to use automation software in addition to base training on the TEM.
Our SEM service offers a high-performance microscope for fast characterization and 3D imaging of fine surface structures on both small and large samples. We offer training and full service imaging for internal and external SEM customers. SEM operation may be conducted by individual investigators or provided by our staff.
Examples of specimens imaged by SEM include:
We offer the following SEM imaging services:
We offer specimen processing for SEM, including:
Our JEOL JSM 6610LV scanning electron microscope has the following features:
Our other SEM equipment includes:
We provide one-on-one training for users interested in learning to operate the JSM6610LV SEM independently. We review sample handling and loading, stage orientation, focusing and image acquisition using the JEOL software. Users can schedule training on our iLab site as assisted use time on the SEM. We grant users who have been cleared as independent by EM shared resource staff 24/7 access to our microscope.