As a lab within the Cooperative Center of Excellence in Hematology (CCEH) at Fred Hutch, Hematopoietic Cell Procurement and Processing is recognized as a leader in the field of acquisition, analysis, and enrichment of normal hematopoietic cells, this facility provides well defined and highly purified populations of cells to support the research of both internal and external investigators.
Complete blood counts (CBCs with +/- differential) are performed using an automated hematology analyzer, the Siemens Advia 2120i Hematology System, with multispecies package (humans and animals). It permits high throughput accurate assessment of 23 hematologic parameters for research purposes only.
This service includes:
This service is available 7 days per week including weekends and holidays, limited hours during weekends and holidays.
User fees are designed simply to recover the direct supply costs associated with the procedure. Examples of certain user fee charges are listed below; other functions are available upon request.
Cost Per Sample:
Fax: 206. 667.5978
The Hematopoetic Cell Procurement and Processing core can perform up to 4-color flow cytometry staining and analyses, as well as in-vitro colony-forming assays on research specimens.
The Hematopoietic Cell Procurement and Processing Core can process normal donor, patient and animal specimens for cell enrichment and/or depletion using small-scale Miltenyi AutoMACS or larger-scale CliniMACS magnetic bead technology.
Types of selection options include CD34 progenitor/stem cells, CD3, CD4, CD8, CD25, CD45RA+/- naïve, memory, or regulatory T-cell subsets, CD14 monocyte/dendritic cells, CD19, CD20, CD22 B-cells, and CD3-depleted/CD56+ NK-cells. Other types of enrichment/depletion procedures are available under special request. Selected cells can be provided fresh or cryopreserved.
The CCEH Hematopoietic Cell Procurement and Processing Core maintains and gives access to a Repository of Cryopreserved Specimens consisting of large quantities of PBC components obtained from normal donors or patients with a variety of malignancies and limited quantities of normal bone marrow.
Also available are freshly donated peripheral blood mononuclear cells (PBMC) and/or G-CSF mobilized peripheral blood cells (PBC), which are acquired through our healthy adult donor volunteer recruitment program.
Learn more information about the donor program.
We maintain an inventory of more than 400 vials of enriched 5-10 million CD34 positive cells collected from approximately 3-4 healthy donors each month.
Location: Thomas Building, D2-334
Fred Hutchinson Cancer Center
P.O. Box 19024
Mail Stop: D2-334
Seattle, WA 98109-1024