We offer a complete suite of genomics and bioinformatics services and collaborate with researchers at every step, from technology selection and experimental design to data generation and analysis. In addition to contacting our genomics team, we strongly encourage researchers to consult with bioinformatics staff in the earliest stages of experimental design. Our bioinformaticians are available for one-on-one consultations.
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| Bioinformatics Support (per hour) | $75.00 | $135.00 | View DescriptionStandalone expert bioinformatics analysis support. |
| Genomics Technical Support (per hour) | $58.00 | $102.30 | View DescriptionStandalone expert genomics laboratory support. |
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| Fragment Analysis 1-47 Samples | $2.15 | $3.79 | View DescriptionDNA fragment sizing. |
| Fragment Analysis 48+ Samples | $1.60 | $2.82 | View DescriptionDNA fragment sizing. |
| CE Sequencing Full Service 1-47 Samples | $5.90 | $10.40 | View DescriptionUsers provide template mixed with primers and Genomics performs PCR, cleanup, and sequencing reactions. |
| CE Sequencing Full Service 48+ Samples | $5.30 | $9.50 | View DescriptionUsers provide template mixed with primers and Genomics performs PCR, cleanup, and sequencing reactions. |
| CE Sequencing Ready to Go 1-47 Samples | $4.00 | $7.05 | View DescriptionUsers perform PCR and Genomics performs cleanup and sequencing reactions. |
| CE Sequencing Ready to Go 48+ Samples | $3.20 | $5.70 | View DescriptionUsers perform PCR and Genomics performs cleanup and sequencing reactions. |
| Cell Line Authentication | $40.00 | $75.00 | View DescriptionSTR profiling of 16 loci using the ThermoFisher CLA IdentiFiler Plus PCR Amplification Kit. |
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| Illumina NovaSeq X Plus | |||
| 10B Lane | $1,450.00 | $2,552.00 | View DescriptionNominal output of 1.25-1.5B read pairs. Up to PE151 reads with up to 10 bp index reads. Custom primers not supported. |
| 25B Lane | $2,400.00 | $4,224.00 | View DescriptionNominal output of 3.1-4B read pairs. Up to PE151 reads with up to 10 bp index reads. Custom primers not supported. |
| 1.5B-100 | $3,700.00 | $6,512.00 | View DescriptionNominal output of 1.6B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 1.5B-200 | $4,150.00 | $7,305.00 | View DescriptionNominal output of 1.6B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 1.5B-300 | $4,350.00 | $7,656.00 | View DescriptionNominal output of 1.6B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 10B-100 | $8,750.00 | $15,400.00 | View DescriptionNominal output of 10-12B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 10B-200 | $10,500.00 | $18,480.00 | View DescriptionNominal output of 10-12B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 10B-300 | $11,450.00 | $20,152.00 | View DescriptionNominal output of 10-12B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 25B-100 | $14,500.00 | $25,520.00 | View DescriptionNominal output of 25-30B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 25B-200 | $17,503.00 | $30,805.00 | View DescriptionNominal output of 25-30B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 25B-300 | $19,050.00 | $33,528.00 | View DescriptionNominal output of 25-30B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| Illumina NextSeq 2000 | |||
| P1-100 | $1,648.00 | $2,472.00 | View DescriptionNominal output of 100M read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P1-300 | $2,000.00 | $3,010.00 | View DescriptionNominal output of 100M read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P1-600 | $2,620.00 | $3,940.00 | View DescriptionNominal output of 100M read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P2-100 | $2,163.00 | $3,245.00 | View DescriptionNominal output of 400M read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P2-200 | $2,600.00 | $3,900.00 | View DescriptionNominal output of 400M read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P2-300 | $3,000.00 | $4,500.00 | View DescriptionNominal output of 400M read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P2-600 | $4,000.00 | $6,000.00 | View DescriptionNominal output of 400M read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P3-100 | $2,600.00 | $3,900.00 | View DescriptionNominal output of 1.1B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P3-200 | $3,000.00 | $4,500.00 | View DescriptionNominal output of 1.1B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P3-300 | $3,150.00 | $4,725.00 | View DescriptionNominal output of 1.1B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P4-50 | $3,130.00 | $4,695.00 | View DescriptionNominal output of 1.8B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P4-100 | $3,400.00 | $5,100.00 | View DescriptionNominal output of 1.8B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P4-200 | $4,000.00 | $6,000.00 | View DescriptionNominal output of 1.8B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| P4-300 | $4,200.00 | $6,300.00 | View DescriptionNominal output of 1.8B read pairs. Reagent kits available for 100, 200, and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| Illumina MiSeq i100 Plus | |||
| 5M_300 | $725.00 | $1,276.00 | View DescriptionNominal output of 5M read pairs. Reagent kits available for 300 and 600 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 5M_600 | $975.00 | $1,716.00 | View DescriptionNominal output of 5M read pairs. Reagent kits available for 300 and 600 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 25M_100 | $850.00 | $1,496.00 | View DescriptionNominal output of 25M read pairs. Reagent kits available for 100, 300, and 600 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 25M_300 | $1,250.00 | $2,200.00 | View DescriptionNominal output of 25M read pairs. Reagent kits available for 100, 300, and 600 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 25M_600 | $1,450.00 | $2,552.00 | View DescriptionNominal output of 25M read pairs. Reagent kits available for 100, 300, and 600 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 50M_100 | $1,100.00 | $1,936.00 | View DescriptionNominal output of 50M read pairs. Reagent kits available for 100, 300, and 600 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 50M_300 | $1,475.00 | $2,596.00 | View DescriptionNominal output of 50M read pairs. Reagent kits available for 100, 300, and 600 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 50M_600 | $1,850.00 | $3,256.00 | View DescriptionNominal output of 50M read pairs. Reagent kits available for 100, 300, and 600 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 100M_100 | $1,375.00 | $2,420.00 | View DescriptionNominal output of 100M read pairs. Reagent kits available for 100 and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| 100M_300 | $1,850.00 | $3,256.00 | View DescriptionNominal output of 100M read pairs. Reagent kits available for 100 and 300 cycles. Dedicated flow cells can be run in any configuration and may use custom primers. |
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| 16S Sequencing - Per Sample | $60.00 | $105.50 | View DescriptionTargets the V3/V4 hypervariable region using the Zymo Research Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI). |
| AutoCUT&RUN (Up to 20 samples) | $1,900.00 | $3,344.00 | View DescriptionAutomated CUT&RUN library preparation service. Available in 1/4-, 1/2-, and full-plate scales Users must contact Genomics to schedule a run prior to submitting samples. |
| AutoCUT&RUN (Up to 44 samples) | $3,708.00 | $6,527.00 | View DescriptionAutomated CUT&RUN library preparation service. Available in 1/4-, 1/2-, and full-plate scales Users must contact Genomics to schedule a run prior to submitting samples. |
| AutoCUT&RUN (Up to 92 samples) | $6,922.00 | $12,138.00 | View DescriptionAutomated CUT&RUN library preparation service. Available in 1/4-, 1/2-, and full-plate scales Users must contact Genomics to schedule a run prior to submitting samples. |
| AutoCUT&Tag (Up to 20 samples) | $1,030.00 | $1,813.00 | View DescriptionAutomated CUT&Tag library preparation service. Available in 1/4-, 1/2-, and full-plate scales Users must contact Genomics to schedule a run prior to submitting samples. |
| AutoCUT&Tag (Up to 44 samples) | $1,545.00 | $2,720.00 | View DescriptionAutomated CUT&Tag library preparation service. Available in 1/4-, 1/2-, and full-plate scales Users must contact Genomics to schedule a run prior to submitting samples. |
| AutoCUT&Tag (Up to 92 samples) | $2,472.00 | $4,351.00 | View DescriptionAutomated CUT&Tag library preparation service. Available in 1/4-, 1/2-, and full-plate scales Users must contact Genomics to schedule a run prior to submitting samples. |
| mRNA-seq Library Preparation and Sequencing | $150.00 | $264.00 | View DescriptionBulk RNA-seq using poly-A selection. Requires high-quality RNA (RIN≥8) as input. Service includes library preparation and sequencing to ≥30M PE50 reads. |
| Total RNA-seq Library Preparation and Sequencing | $250.00 | $440.00 | View DescriptionBulk RNA-seq using ribodepletion and random priming. Compatible with degraded or FFPE (DV200 ≥ 30) RNA. Service includes library preparation and sequencing to ≥60M PE50 reads. |
| NexteraXT | $40.00 | $70.40 | View DescriptionTagmentation-based library preparation using the Illumina NexteraXT DNA Prep. Ideal for microbial genomes or amplicons. |
| Whole Genome Sequencing (1-47 samples) | $155.00 | $273.00 | View DescriptionWhole Genome Sequencing library preparation. Kits available include the Illumina DNA PCR-Free prep for intact genomic DNA and the IDT xGen cfDNA & FFPE Library Kit for degraded, FFPE, or cfDNA. |
| Whole Genome Sequencing (48+ samples) | $75.00 | $150.00 | View DescriptionWhole Genome Sequencing library preparation. Kits available include the Illumina DNA PCR-Free prep for intact genomic DNA and the IDT xGen cfDNA & FFPE Library Kit for degraded, FFPE, or cfDNA. |
| Methylation Sequencing (1-47 samples) | $155.00 | $273.00 | View DescriptionWhole Genome Methylation Sequencing library preparation using the NEB EM-seq v2 kit. |
| Methylation Sequencing (48+ samples) | $75.00 | $150.00 | View DescriptionWhole Genome Methylation Sequencing library preparation using the NEB EM-seq v2 kit. |
| Whole Exome Sequencing (1-8 samples) | $2,430.00 | $4,277.00 | View DescriptionWhole Exome Sequencing library preparation and capture using the Twist Exome 2.0 panel. |
| Whole Exome Sequencing (9-16 samples) | $2,970.00 | $5,228.00 | View DescriptionWhole Exome Sequencing library preparation and capture using the Twist Exome 2.0 panel. |
| Whole Exome Sequencing (17-24 samples) | $3,615.00 | $6,363.00 | View DescriptionWhole Exome Sequencing library preparation and capture using the Twist Exome 2.0 panel. |
| Whole Exome Sequencing (25-48 samples) | $6,740.00 | $11,863.00 | View DescriptionWhole Exome Sequencing library preparation and capture using the Twist Exome 2.0 panel. |
| Whole Exome Sequencing (49-96 samples) | $10,590.00 | $18,639.00 | View DescriptionWhole Exome Sequencing library preparation and capture using the Twist Exome 2.0 panel. |
| Custom Targeted Sequencing Applications | Inquire | Inquire | View DescriptionGenomics supports a variety of custom targeted sequencing applications including the ArcherDx suite of multiplexed amplicon panels as well as custom or off-the-shelf capture panels from IDT or Twist Bio. |
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| 10x Genomics Chromium X Training | $635.00 | $1,118.00 | View DescriptionTraining for walk-up useage of 10x Genomics Chromium X Controller. |
| 10x Genomics Chromium X Use | $206.00 | $412.00 | View Description(Per chip run) Walk-up usage of 10x Genomics Chromium X Controller. |
| 10x Genomics Full Service GEM/cDNA Preparation | $75.00 | $133.00 | View DescriptionFull-service 10x Genomics GEM/cDNA and/or library preparation. Users are required to contact Genomics for consultation and to schedule a run prior to submitting samples. Users are required to provide all reagents from 10x Genomics. |
| 10x Genomics Full Service Library Preparation | $210.00 | $370.00 | View DescriptionFull-service 10x Genomics GEM/cDNA and/or library preparation. Users are required to contact Genomics for consultation and to schedule a run prior to submitting samples. Users are required to provide all reagents from 10x Genomics. |
| Takara Bio SMART-Seq Pro | $3,500.00 | $6,160.00 | View Description(Per chip run) Full service library preparation using the Takara Bio SMART-Seq Pro Application kit. Users are required to contact Genomics for consultation and to schedule a run prior to submitting samples. |
| Takara Bio Shasta Total RNA | $4,500.00 | $7,920.00 | View Description(Per chip run) Full service library preparation using the Takara Bio Shasta Total RNA kit. Users are required to contact Genomics for consultation and to schedule a run prior to submitting samples. |
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| Spatial Library Preparation | Inquire | Inquire | View DescriptionIn collaboration with the Experimental Histopathology Shared Resource (exphiso@fredhutch.org), we support the 10x Genomics Visium platform. Users are required to contact spatial@fredhutch.org for consultation and to schedule a run prior to submitting samples. |
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| PromethION sequencing (Flow cell) | $1,100.00 | $1,936.00 | View DescriptionDedicated PromethION flow cell. Can be washed and stored as needed to enable repeat usage. |
| PromethION sequencing (P2 Solo access and loading) | $100.00 | $176.00 | View DescriptionAssesed each time a dedicated PromethION flow cell is loaded. |
| PromethION Flow Cell Wash & Store | $40.00 | $71.00 | View DescriptionWash and store of PromethION flow cells for repeat use. |
| Whole Plasmid Sequencing (per sample) | $15.00 | $26.35 | View DescriptionWhole plasmid sequencing. Outputs include up to 5,000 reads, assembled plasmid sequence, and annotations. |
| Rapid Barcoding Library Preparation (per sample) | $50.00 | $88.00 | View DescriptionTagmentation-based library prep. Suitable for microbial genomes and amplicons. |
| Native Barcoding Library Preparation (per batch of ≤24 samples) | $300.00 | $528.00 | View DescriptionLigation-based library prep. Suitable for mammalian genomes, amplicons, and long-read RNA sequencing. |
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| SMRT Bell Library Prep | $335.00 | $590.00 | View DescriptionStandard SMRT Bell Prep for amplicons or genomic DNA. |
| PacBio Kinnex Lib Prep | $639.00 | $1,125.00 | View DescriptionKinnex library prep including single-cell RNA, bulk RNA, and 16S amplicons. |
| PacBio Revio Sequencing | Inquire | Inquire | View DescriptionWe collaborate with Seattle Children's to enable PacBio Revio Sequencing. |
| Service | Internal / Consortium User Fee | External User Fee | Description |
|---|---|---|---|
| Covaris DNA/RNA Shearing (Sample) | $12.00 | $21.10 | View DescriptionNucleic acid shearing using Covaris LE220 Plus. |
| Covaris DNA/RNA Shearing (Plate) | $660.00 | $1,162.00 | View DescriptionNucleic acid shearing using Covaris LE220 Plus. |
| TapeStation Analysis | $8.50 | $15.00 | View DescriptionNucleic acid sizing. |
| Illumina Library qPCR | $35.00 | $61.60 | View DescriptionIllumina library quantification using KAPA Library Quantification Kit. |
| Qubit Analysis | $10.00 | $17.60 | View DescriptionFluorometic nucleic acid quantification. |
| QuantStudio 5 Use (per hour) | $12.00 | $21.15 | View DescriptionWallk up use of QuantStudio5 Real Time PCR Instruments. |
Bioinformatics Team Contact
Matt Fitzgibbon
Director, Bioinformatics