The genomics team provides expertise to support the generation of genomics-based data. Our specialized labs and experienced staff offer a full suite of genomics services. We partner with the bioinformatics team to provide a streamlined set of complete services.
The genomics team, in partnership with our bioinformatics colleagues, provides end-to-end support of Illumina next-generation sequencing projects. Our integrated resources are here for you every step of the way, from experimental design to library prep and downstream analysis.
The sequencers we have to support this work include an Illumina NovaSeq 6000, a NextSeq 2000, a HiSeq 2500, and two MiSeqs. We offer a multitude of library preps including, but not limited to, bulk RNA-seq, single-cell RNA-seq (10x Genomics), RNA exome, polysome profiling, whole exome and whole genome sequencing, cell-free DNA, and targeted amplicon sequencing.
We also offer Cleavage Under Targets and Release Using Nuclease (CUT&RUN), an antibody-targeted chromatin profiling method developed by the Hutch’s Henikoff Lab to examine genome-wide occupancy of transcription factors and chromatin modifying proteins. CUT&RUN has been automated as AutoCUT&RUN, and we offer sample-to-Illumina library processing using a Beckman Biomek FX liquid-handling robot to facilitate high-throughput processing of 96 samples in two days.
Our experienced team will also work with you to develop and implement custom sequencing approaches.
The PacBio Sequel is a third-generation sequencing platform that employs Single Molecule, Real-Time (SMRT) Sequencing technology to provide real-time single-molecule sequencing without the need for sample amplification. The Sequel can deliver up to 500K-long reads with high consensus accuracy and is ideal for de novo genome sequencing, single-base modification detection, and pooled amplicon sequencing. We offer both SMRTbell library preparation and sequencing services.
We provide both full service and ready-to-go capillary electrophoresis-based sequencing, along with fragment and SNP analysis. Genetic analysis is performed using two staff-operated Applied Biosystems (ABI) 3730xl DNA Analyzers.
Genomics offers qualitative and semi-quantitative analysis of DNA and RNA using the Agilent 4200 Tapestation.
The genomics team provides access to self-service qPCR (real-time PCR) instruments located in DE-341. Four ABI QuantStudio5 Real Time PCR Systems (two 96-well blocks and two 384-well blocks) are available 24 hours a day, with staff available for instrument training and to answer questions Monday through Friday, 9 a.m. to 5 p.m. To schedule time, please access the instructions below.
We offer digital gene-expression analysis using a staff-operated Nanostring nCounter automated system. The flexible system detects RNA, miRNA and DNA from a variety of sample types, including FFPE and whole-cell lysates. Both catalog and custom Codesets can be used.
We provide material support, consultation, and training in the use of gene modulation technology and precision, high-throughput liquid handling systems with applications ranging from high-throughput screens to investigating individual gene function and custom protocol development.