Fred Hutch Clinical Test Menu

Fred Hutch CLIA Test Menu

 

Test Menu Synonyms Indications Methodology Sample Type Preservative Specimen Acceptibility Requirements Turnaround Time
FLT3-ITD with Allelic Ratio FMS-like tyrosine kinase 3, FLT, Internal Tandem Duplication, ITD AR AML PCR BMA, tissue or PB >20% blasts EDTA Must be received in lab within 48 hours of draw 2-3 business days
FLT3-TKD FLT3 D835/I836, FLT3 point mutation AML PCR BMA, tissue or PB >20% blasts EDTA Must be received in lab within 48 hours of draw 2-4 business days
NPM1 NPM, NPM-1, Nucleophosmin AML PCR BMA, tissue or PB >20% blasts EDTA Must be received in lab within 48 hours of draw 2-4 business days
CEBPα CCAAT/enhancer binding protein alpha, CEBP, CEBP-alpha AML PCR BMA, tissue or PB >20% blasts EDTA Must be received in lab within 48 hours of draw 2-4 business days
IDH2 Isocitrate Dehydrogenase 2, R140 & R172 mutations AML Sanger Sequencing BMA, tissue or PB >20% blasts EDTA Must be received in lab within 48 hours of draw 3-5 business days
p210 BCR/ABL BCR-ABL1, p210, QP210, Quantitative BCR/ABL, t(9;22) CML,          Ph + ALL QPCR PB EDTA Must be received in lab within 48 hours of draw 1-2 business days
ABL Kinase Mutational Analysis ABL mutation screening, ABL sequencing, TKI resistance testing, T315I, ABLKM CML,                 Ph + ALL Sanger Sequencing PB or BMA EDTA Must be received in lab within 48 hours of draw 4-5 business days
PD-L1 IHC Programmed Death-Ligand 1; CD274; clone 22C3 Various Immunohistochemistry Wet tissue in 10%NBF Or paraffin block Or 3-4 unstained FFPE slides 10% neutral buffered formalin N/A 3-5 business days
CD56 IHC NCAM1 Various Immunohistochemistry Wet tissue in 10%NBF Or paraffin block Or 3-4 unstained FFPE slides 10% neutral buffered formalin N/A 3-5 business days
CK20 IHC keratin 20 Various Immunohistochemistry Wet tissue in 10%NBF Or paraffin block Or 3-4 unstained FFPE slides 10% neutral buffered formalin N/A 3-5 business days
Merkel Cell Polyoma virus IHC CM2B4 MCC Immunohistochemistry Wet tissue in 10%NBF Or paraffin block Or 3-4 unstained FFPE slides 10% neutral buffered formalin N/A 3-5 business days
H&E Stain Hematoxylin and Eosin Various Routine staining Wet tissue in 10%NBF Or paraffin block Or 3-4 unstained FFPE slides 10% neutral buffered formalin N/A 3-5 business days
Custom Non-disclosable IHC selection assays study specific targets Various Immunohistochemistry Wet tissue in 10%NBF Or paraffin block Or 3-4 unstained FFPE slides 10% neutral buffered formalin N/A 3-5 business days
HER2 quantitative ERBB2 expression levels in cancer immuno-MRM Unstained FFPE tissue on slides FFPE N/A 2 weeks

Additional Services

Molecular Exploratory Markers

Assay Synonyms Methodology
WT1 Wilms' Tumor 1 QPCR: absolute quantification
AKR1C3 Aldo-Keto Reductase Family 1 Member C3 QPCR: relative quantification

Targeted Proteomics Exploratory Markers

Please go to the Paulovich Lab website for a full list of markers.

Routine Histology Services

Service Details Methodology
FFPE processing Creation of Formalin Fixed paraffin embedded tissue blocks Routine
Paraffin and frozen sectioning Microtomy Routine
Gross Examination Tissue dissection, gross review May be performed by board-certified pathologist
Custom IHC assay validation  optimization & validation of custom assays Immunohistochemistry