Cryo-EM

Cutting-Edge Technique to Determine Molecular Structures

The Cryo-EM core is Fred Hutch’s newest shared resource. We aim to bring a new structural determination technique to support users seeking to solve difficult molecular structures at an ultrafine scale. Recent advances in cryo-EM technology and software algorithms have made it a compelling structural biology method for flexible proteins that resist visualization by other techniques.

We offer training in all steps of the cryo-EM process, from sample preparation to image processing. All users will be supported by experienced staff members and will be granted 24/7 access to the microscopes and sample preparation instruments after they have satisfied training requirements. Our instruments, training and services are available to both internal and external users.

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Sample Preparation

We train users to generate their own cryo-EM and negative-stain grids, as well as offering sample preparation services. Core staff members are available to troubleshoot challenges and offer guidance on experimental conditions.

View our Sample Preparation Services
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Data Collection and Processing

The core offers access to two transmission electron microscopes. We train users to collect and process their own high-resolution data, as well as offering data collection and processing services. Core staff members are also available to consult during project development.

View our Data Collection and Processing Services

Our Team at Work

Our expert staff is available for training and consultation for new and experienced cryo-EM users alike. We provide services in sample preparation, data collection and data processing, and we aim to empower researchers seeking to generate and analyze their own structural data.

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Cryo-TEM Scopes
We have two top-of-the-line cryogenic transmission electron microscopes.

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Å Resolution
Our technologies can determine structures at close to one angstrom resolution.

1500

Frames per Second
The K3 direct detector's maximum data-recording rate reaches 1500 frames per second.

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A6000 GPUs
Our powerful processing units support on-the-fly drift correction and processing.

How to Reach Us

Caleigh Azumaya

Caleigh Azumaya, Ph.D.

Facility Manager, Cryo-EM shared resource
Phone: 206.667.3176
Work Location: A3-205