Recent Publications
The publications below reflect recent research that has been supported by the products and services provided by the Fred Hutch CCEH Resource Cores.
Huang P, Peslak SA, Shehu V, et al. let-7 miRNAs repress HIC2 to regulate BCL11A transcription and hemoglobin switching. Blood. 2024;143(19):1980-91. PubMed PMID: 38364109.
Do BT, Hsu PP, Vermeulen SY, et al. Nucleotide depletion promotes cell fate transitions by inducing DNA replication stress. Dev Cell. 2024. PubMed PMID: 38823395.
Kanke KL, Rayner RE, Abel E, et al. Single-Stranded DNA with Internal Base Modifications Mediates Highly Efficient Gene Insertion in Primary Cells. bioRxiv. 2024. PubMed PMID: 38352420.
Lane DD, Gottimukkala KSV, Cunningham RA, et al. Cas9 RNP Physiochemical Analysis for Enhanced CRISPR-AuNP Assembly and Function. bioRxiv. 2024. PubMed PMID: 38617334.
Levesque S, Cosentino A, Verma A, et al. Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism. Nat Biotechnol. 2024. PubMed PMID: 38806736.
Xi C, Palani C, Takezaki M, et al. Simvastatin-Mediated Nrf2 Activation Induces Fetal Hemoglobin and Antioxidant Enzyme Expression to Ameliorate the Phenotype of Sickle Cell Disease. Antioxidants (Basel). 2024;13(3). PubMed PMID: 38539870.
Yan J, Oyler-Castrillo P, Ravisankar P, et al. Improving prime editing with an endogenous small RNA-binding protein. Nature. 2024;628(8008):639-47. PubMed PMID: 38570691.
Arora S, Szulzewsky F, Jensen M, et al. Visualizing genomic characteristics across an RNA-Seq based reference landscape of normal and neoplastic brain. Sci Rep. 2023;13(1):4228. PubMed PMID: 36918656.
Boontanrart MY, Mächler E, Ponta S, et al. Engineering of the endogenous HBD promoter increases HbA2. Elife. 2023;12. PubMed PMID: 37265399.
Gnanapragasam MN, Planutis A, Glassberg JA, et al. Identification of a genomic DNA sequence that quantitatively modulates KLF1 transcription factor expression in differentiating human hematopoietic cells. Sci Rep. 2023;13(1):7589. PubMed PMID: 37165057.
Hsieh E, Janssens DH, Paddison PJ, et al. A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latency. PLoS Pathog. 2023;19(1):e1011101. PubMed PMID: 36706161.
Kinsella S, Evandy CA, Cooper K, et al. Damage-induced pyroptosis drives endog thymic regeneration via induction of Foxn1 by purinergic receptor activation. bioRxiv. 2023. PubMed PMID: 36711570.
Kuppers DA, Linton J, Ortiz Espinosa S, et al. Gene knock-outs in human CD34+ hematopoietic stem and progenitor cells and in the human immune system of mice. PLoS One. 2023;18(6):e0287052. PubMed PMID: 37379309.
Li C, Georgakopoulou A, Newby GA, et al. In vivo HSC prime editing rescues sickle cell disease in a mouse model. Blood. 2023;141(17):2085-99. PubMed PMID: 36800642.
Li C, Shin H, Bhavanasi D, et al. Expansion of human hematopoietic stem cells by inhibiting translation. bioRxiv. 2023. PubMed PMID: 38077058.
Martin-Rufino JD, Castano N, Pang M, et al. Massively parallel base editing to map variant effects in human hematopoiesis. Cell. 2023;186(11):2456-74.e24. PubMed PMID: 37137305.
Neugebauer ME, Hsu A, Arbab M, et al. Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity. Nat Biotechnol. 2023;41(5):673-85. PubMed PMID: 36357719.
Qin K, Lan X, Huang P, et al. Molecular basis of polycomb group protein-mediated fetal hemoglobin repression. Blood. 2023;141(22):2756-70. PubMed PMID: 36893455.
Rotolo A, Whelan EC, Atherton MJ, et al. Unedited allogeneic iNKT cells show extended persistence in MHC-mismatched canine recipients. Cell Rep Med. 2023;4(10):101241. PubMed PMID: 37852175.
Schuster SL, Arora S, Wladyka CL, et al. Multi-level functional genomics reveals molecular and cellular oncogenicity of patient-based 3' untranslated region mutations. Cell Rep. 2023;42(8):112840. PubMed PMID: 37516102.
Sporrij A, Choudhuri A, Prasad M, et al. PGE(2) alters chromatin through H2A.Z-variant enhancer nucleosome modification to promote hematopoietic stem cell fate. Proc Natl Acad Sci U S A. 2023;120(19):e2220613120. PubMed PMID: 37126722.
Vamva E, Ozog S, Leaman DP, et al. A lentiviral vector B cell gene therapy platform for the delivery of the anti-HIV-1 eCD4-Ig-knob-in-hole-reversed immunoadhesin. Mol Ther Methods Clin Dev. 2023;28:366-84. PubMed PMID: 36879849.
Voit RA, Tao L, Yu F, et al. A genetic disorder reveals a hematopoietic stem cell regulatory network co-opted in leukemia. Nat Immunol. 2023;24(1):69-83. PubMed PMID: 36522544.
Wang H, Georgakopoulou A, Zhang W, et al. HDAd6/35++ - A new helper-dependent adenovirus vector platform for in vivo transduction of hematopoietic stem cells. Mol Ther Methods Clin Dev. 2023;29:213-26. PubMed PMID: 37081854.
Weinstock JS, Gopakumar J, Burugula BB, et al. Aberrant activation of TCL1A promotes stem cell expansion in clonal haematopoiesis. Nature. 2023;616(7958):755-63. PubMed PMID: 37046083.
Xi C, Pang J, Zhi W, et al. Nrf2 sensitizes ferroptosis through l-2-hydroxyglutarate-mediated chromatin modifications in sickle cell disease. Blood. 2023;142(4):382-96. PubMed PMID: 37267508.
Yin M, Izadi M, Tenglin K, et al. Evolution of nanobodies specific for BCL11A. Proc Natl Acad Sci U S A. 2023;120(3):e2218959120. PubMed PMID: 36626555.
Zhao J, Jia Y, Mahmut D, et al. Human hematopoietic stem cell vulnerability to ferroptosis. Cell. 2023;186(4):732-47.e16. PubMed PMID: 36803603.