Expanding on the Cooperative Center of Excellence in Hematology (CCEH) at Fred Hutchinson Cancer Center’s expertise in providing Human CD34+ HSPCs, we have established a high-efficiency and high-viability protocol for gene editing in CD34+ cells. We are offering a Gene Editing Service (Basic) in conjunction with provision of donor-derived CD34+ HSPCs. Using non-viral delivery of Cas9:sgRNA ribonucleoprotein complexes, we generate high efficiency biallelic single, double and triple gene Knock Outs (KO) in human CD34+ cells. On a case-by-case basis we are also providing custom editing services not limited to but covering the use of alternative Cas systems, targeted insertion and library screens.
Data from Kuppers et al. 2023
We've optimized this technique to minimize time in culture, and, as a result, cells maintain multi-lineage potential in vitro and in vivo.
2. Design of sgRNAs
3. Selection of appropriate controls
4. KO procedure
5. Sequencing-based validation of targeting event
6. Provision of viably frozen KO CD34+ cells along with donor-matched controls cells
Please send a service request through the contact link below and we will get back to you about scheduling a consultation.
In addition to the services covered in the basic service, custom editing services will provide closer collaboration to tailor editing to your experimental needs. What is covered will vary on a case-by-case basis but may include things such as alternative Cas systems, targeted insertion, library screens or additional protocol development and optimization. Please send us a description of your project through the link below and we will get back to you about our ability to support your needs and to schedule a consultation.
Base Gene Editing Service