Science Spotlight

Why Helicobacter pylori needs to stay in shape

One of the greatest controversies and most surprising discoveries in modern medicine was that stomach ulcers are caused by the bacterium Helicobacter pylori. Thanks to Drs. Robin Warren and Barry Marshall, it is now well-established that H. pylori colonizes in the viscous epithelial mucus layer of the human stomach, which protects both the underlying epithelial cells and H. pylori from the stomach lumen. Indeed, H. pylori can survive direct exposure to stomach acid for only minutes. Yet despite the advent of antibiotics, H. pylori persists in approximately one-half of the world’s population and leads to severe complications, including stomach cancer, in 15-20% of chronically infected individuals.


To access and maintain its niche, H. pylori moves via flagella in response to chemotactic signals. It has been proposed that the helical shape of H. pylori also contributes to its motility in viscous mucus; however, this particular aspect has never been directly assessed. Therefore, former graduate student Dr. Laura Sycuro, her advisor Dr. Nina Salama of the Human Biology Division, and colleagues made a series of H. pylori mutants with altered morphology to evaluate whether its helical shape enables it to traverse viscous solutions and thus colonize stomach mucosa.


Salama and colleagues previously generated a library of H. pylori mutants, using transposons to randomly disrupt H. pylori genes, and sequencing to locate the resulting disruptions. Sycuro et al. screened this library for morphology changes by light microscopy and identified two mutants that yielded predominantly straight rod-shaped bacteria (disruption in Csd5 gene), and exclusively straight rod-shaped bacteria (disruption in Csd4 gene).


Whilst both mutants were unaffected in terms of their ability to grow in vitro and withstand extreme osmotic stress, they did exhibit significantly reduced motility in soft agar compared to wild-type H. pylori. In addition, the Csd4 mutant exhibited decreased colonization of murine stomachs (n.b., the Csd5 mutant was not analyzed due to its heterogeneous shape phenotype). The Salama Lab has ongoing efforts to evaluate whether the soft agar results will translate with epithelial mucus and to understand how Csd4 and Csd5 fit into a larger network of genes that regulate cell shape. Nonetheless, the results of this study go a long way to support the hypothesis that the helical shape of H. pylori is indeed integral to its motility and niche acquisition.


Sycuro LK, Wyckoff TJ, Biboy J, Born P, Pincus Z, Vollmer W, Salama NR. 2012. Multiple peptidoglycan modification networks modulate Helicobacter pylori’s cell shape, motility, and colonization potential. PLoS Pathogens 8:e1002603.


There are no tags on this page. A list of tags will appear here once there are.