To investigate the possible tumor suppressor role of a cell junction protein in EAC, Drs. Andrew Kaz and William Grady and colleagues in the Clinical Research Division report on the methylation, expression and role of PKP1 in progression of BE to EAC. PKP1 encodes a desmosomal protein that links cadherins to intermediate filaments within the cell. Thus, PKP1 plays a critical role in cell-cell adhesive junctions, including those found in normal esophageal epithelium. PKP1 has also been implicated as a tumor suppressor in other epithelial cancers. RT-PCR and immunohistochemical staining showed significantly reduced expression of PKP1 in primary tissues from patients with BE, high-grade dysplasia or EAC relative to normal tissue. A significant increase in the CpG methylation of the promoter of PKP1 was also observed in high-grade dysplasia and EAC relative to normal or Barrett’s epithelium. Initial loss of PKP1 expression in BE may be due to methylation at other sites in the PKP1 locus or non-methylation factors. During the transition of BE to EAC, CpG methylation is likely to reinforce’ this decreased expression of PKP1. Finally, siRNA-mediated knock-down of PKP1 in two esophageal cell lines that normally express PKP1 resulted in dramatic increases in cell motility.
Thus, factors that down-regulate PKP1 expression, including CpG methylation, contribute to disrupted cell-cell adherence and BE progression. Further identification of the initial factors that down-regulate PKP1 expression, as well as the molecular mechanisms behind increased motility will further our understanding of EAC development.
Kaz AM, Luo Y, Dzieciatkowski S, Chak A, Willis JE, Upton MP, Leidner RS, Grady WM. Aberrantly methylated PKP1 in the progression of Barrett’s esophagus to esophageal adenocarcinoma. Genes, Chromosomes and Cancer. DOI: 10.1002/gcc.21923.