International Histocompatibility Working Group
Photo by Emil Madraimov / IHWG
The IHWG reference panels have been compiled to represent a diversity of genes based on population demographics (ethnicities, world regions, relatives) or MHC region characteristics (DQ/DP, MICA, homozygosity, polymorphism, etc.). Each panel varies in number of samples and each sample contains 2ug of purified DNA. Larger microgram amounts are available upon request.
The Consanguineous Reference Panel is composed of 49 cell lines from the 10th workshop which were indicated to be HLA homozygous by descent.
The Anthropology Reference Panel is composed of 15 samples from different regions of the world.
The NK/KIR Phase I Reference Panel is composed of 12 CEPH family groups. Each family group includes a father, mother, and two children. KIR genotypes and KIR haplotypes were identified for each DNA sample during the 13th workshop. The 48 samples were typed using PCR-SSP and PCR-SSOP based methods.
The KIR Phase II Reference Panel consists of a reduced number of samples which are included in the larger NK/KIR Phase I reference panel. NK/KIR Phase II has 4 reference families that make up the 16 samples.
The DQ/DP Reference Panel is a compilation of 41 DNA samples that focus on the DQA1, DQB1, DPA1, and DPB1 regions.
The Major Histocompatibility Complex Class I Chain-Related Gene A (MICA) Reference Panel is derived from 39 samples studied at the 13th workshop in the disease division studying innate immune responses.
Samples have been retyped by three independent laboratories in 2016 and 2017 using Next-Generation Sequencing (NGS).
The Sequence Polymorphism (SP) Reference Panel is a combination of 51 DNA samples typed using the highest frequency sequence specific methods possible at the time of the 13th workshop.