SPEAKER: Pavitra Roychoudhury, PhD
TITLE: HIV integration site landscape in long-term ART-suppressed individuals
ABSTRACT: HIV cure strategies rely on accurate measures of the size and composition of the latent reservoir. Integration sites are heritable, measurable markers of reservoir size and clonality, and previous studies have shown repeated HIV integration into specific genes. However, large-scale studies are needed in order to link integration site landscapes to clinical outcomes, and to investigate possible mechanisms of reservoir maintenance. We performed an in-depth analysis of the latent reservoir of CD4+ T cells from 50 HIV-infected individuals receiving long-term antiretroviral therapy using high-throughput assays for integration sites and reservoir size. In this talk I will describe some results from this ongoing project including the distribution of integration sites throughout the genome, relationship with chromatin accessibility, person-to-person variability in the number and abundance of integration sites, and estimated proportion of intact provirus in the reservoir.