This kit contains everything needed for students to transform E. coli with one of our two plasmids, pBLU or pFLO. pBLU is a plasmid that contains the gene for beta-galactosidase, that when expressed and combined with X-Gal is used f as a visual indicator of a functional lacZ transformation. pFLO contains a type of Green Fluorescent Protein (GFP) gene that when expressed by E. coli will make them glow under blue LED or UV light. pFLO comes in 5 different colors and does not need X-Gal.
In this lab, students transform E. coli with either the pBLU plasmid that contains the gene for beta-galactosidase (also referred to as the lacZ gene),or the pFLO plasmid that contains a gene derivative of GFP (green fluorescent protein).
In this lab, students can use the different colors produced by various pFLO plasmids to “paint” with transformed bacteria,incorporating art into the discussion of gene expression and microbiology.
NOTE: Because of limited staff days at the Hutch, please give us a minimum of two weeks’ advance notice so we can process your request.
Current days for Pick up and Drop Off of SEP items are Tuesdays and Thursdays, 9 a.m. – 5 p.m.
1. Complete Online Demographic Form (only needs to be done once, skip if already filled out) Estimated numbers are OK.
2. Fill out either the SCHOOL-YEAR KIT REQUEST form or SUPPLIES-ONLY form: